Recombinant:Article Title: Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modeling.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-beta IV Tubulin antibody [EPR16776] Abcam Cat#ab179509; RRID: AB_2716759 Anti-Beta-Tubulin IV [ONS1A6] Emergo Biogenex Cat#MU178-UC; RRID: AB_2335625 MUC5AC Monoclonal Antibody (45M) Thermo Fisher Scientific Cat#MA1-38223; RRID: AB_2266697 Anti-p63 antibody [EPR5701] Abcam Cat#ab124762; RRID: AB_10971840 FOXJ1 Monoclonal Antibody (2A5) Thermo Fisher Scientific Cat#14-9965-80; RRID: AB_1548836 SLPI, Human, pAb Hycult Biotech Cat#HP9024; RRID: AB_2286624 Human pIgR Antibody R&D systems Cat#MAB27171; RRID: AB_3657978 Monoclonal Antibody to CC16 Origene Tech Cat#AM26360PU-N; RRID: AB_11216041 Anti-RSV Antibody, clone 133-1H Merck Cat#MAB8262; RRID: AB_95302 Anti-Rabbit IgG, Alexa Fluor 488 Thermo Fisher Scientific Cat#A-11034; RRID: AB_2576217 Anti-Mouse IgG1, Alexa Fluor 647 Thermo Fisher Scientific Cat#A-21240; RRID: AB_2535809 Chemicals and recombinant proteins DMEM Thermo Fisher Scientific Cat#12634-028 Opti-MEM Thermo Fisher Scientific Cat#31985062 BEpiCM-b Sciencell Cat#3211 advanced DMEM/F-12 Thermo Fisher Scientific Cat#12634-028 B-27 Supplement, serum free Thermo Fisher Scientific Cat#17504001 HEPES Thermo Fisher Scientific Cat#15630080 GlutaMAX Thermo Fisher Scientific Cat#35050-061 Hydrocortisone Sigma-Aldrich Cat#H0888 Epinephrine hydrochloride Sigma-Aldrich Cat#E4642 N-acetyl-L-cysteine Sigma-Aldrich Cat#A9165 Nicotinamide Sigma-Aldrich Cat#N0636 3,3′,5-Triiodo-L-thyronine sodium salt Sigma-Aldrich Cat#T6397 Penicillin/streptomycin Thermo Fisher Scientific Cat#15070-063 Primocin InvivoGen Cat#ant-pm-2 Amphotericin B Thermo Fisher Scientific Cat#15290018 Gentamicin Sigma-Aldrich Cat#G1397 Vancomycin Sigma-Aldrich Cat#SBR00001 A83-01 Tocris Cat#2939/10 Y-27632 Selleck Chemicals Cat#S1049 Rapamycin Sigma-Aldrich Cat#553210 DAPT Thermo Fisher Scientific Cat15467109 DMH1 Selleck Chemicals Cat#S7146 TTNPB Cayman Cat#16144-1 RSPO3-Fc Fusion Protein CM U-Protein Express Cat#R001 Recombinant human Heregulin-β1 PeproTech Cat#100-03 Recombinant human FGF10 PeproTech Cat#100-26 Recombinant human HGF PeproTech Cat#100-39H Recombinant human EGF PeproTech Cat#AF-100-15 Recombinant human FGF7 PeproTech Cat#100-19 Recombinant Interleukin-1β PeproTech Cat#200-01 Nirsevimab Provided by AstraZeneca N/A (Continued on next page) e1 Cell Reports Medicine 7, 102692, April 21, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagen IV Sigma-Aldrich Cat#C7521 PureCol Advanced BioMatrix Cat#5005 Cultrex Basement Membrane Extract, Type 2 Trevigen Cat#3532-010 TrypLE express enzyme Thermo Fisher Scientific Cat#12605010 CryoStor CS10 STEMCELL Technologies Cat#07930 SiR-tubulin Spirochrome AG Cat#SC002 DAPI Sigma-Aldrich Cat#D9542 ProLong Gold Thermo Fisher Scientific Cat#P36934 VX-809 Selleck Chemicals Cat#S1565 VX-661 Selleck Chemicals Cat#S7059 VX-445 MedChemExpress Cat#HY-11177 VX-770 Selleck Chemicals Cat#S1144 Calcein green (AM) Invitrogen Cat #C34852 Forskolin Sigma-Aldrich Cat#F3917 G418 (Geneticin) Invivogen Cat#GNL-40-03 ELX-02 (Exaluren) MedChemExpress Cat#HY-114231 Cultureware 96-well culture plates Greiner Bio-One Cat# 655182 24-well culture plates Greiner Bio-One Cat# 662160 12-well culture plates Greiner Bio-One Cat#665165 6-well culture plates Greiner Bio-One Cat#657160 6.5 mm Transwell with 0.4 μm Pore Polyester Membrane Insert Corning Cat#3470 Nunc dishes (35 mm) with UpCell Surface Thermo Fisher Scientific Cat#174904 Commercial kits RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA synthesis kit Bio-Rad Cat#1708891 iQ SYBR Green Supermix Bio-Rad Cat#1708880 Oligonucleotides qPCR Primers This papers, Table S4 N/A Experimental models: Primary cells and cell lines Human Nasal Epithelial Cells (HNEC) UMC Utrecht Protocol ID: 16–586, and 21-044 Human Epithelioma-2 (HEp-2) ATCC Cat#CCL-23 Experimental models: viral strains mKate-RSV-A2 Hotard et al.37 N/A RSV-A and -B clinical isolate strains Langedijk et al.38 N/A Equipment Thunder Imager 3D live Cell with Leica N/A Leica DFC9000 GCT camera Leica N/A Zeiss LSM800 confocal microscope Zeiss N/A Nanodrop spectrophotometer Thermo Fisher N/A CFX96 real-time detection machine Bio-Rad N/A BD FACSJazz BD Bioscience N/A Software and algorithms Leica LAS X Software Leica https://www.leicamicrosystems.com/ Zen Blue Software Zeiss https://www.zeiss.com/ ImageJ/FIJI NIH, Fiji developers https://imagej.net/Fiji/ GNU Image Manipulation Program https://www.gimp.org/ (Continued on next page) Cell Reports Medicine 7, 102692, April 21, 2026 e2
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